Flow Cytometry Leukemia or Lymphoma
Test Code: LAB2297
CPT Code: 88184 001 88185 001 88187 001 88188 001 88189 001
Alert
Bone marrow in EDTA or bone marrow cores should be transported to Flow Cytometry at room temperature within 6 hours. Call Flow Cytometry to alert them.
CSF, body fluids, FNA and tissue should be transported refrigerated or on ice (do not freeze)
Synonyms
Flow Cytometry; Flow Immunophenotyping; Leukemia Profile; Lymphoma Profile; Plasma Cell Neoplasm Profile; Hematoymphoid Neoplasm Profile; Comp panel: Leukemia/Lymphoma; Leukemia/Lymphoma Evaluation; Leukemia/Lymphoma Profile; Leukemia Panel; Lymphoma Panel; Leukemia; Lymphoma; Flow Cytometry Panel; Flow Cytometry Report
Alternate Test ID
480260
Specimen Container
4.0 mL Lavender Top Tube (EDTA) ; Also acceptable: 6.0 mL Dark Green Top Tube (Sodium Heparin); Sterile, Leak-Proof Container ; RPMI + 10% FCS
Minimum Volume
2.0 mL whole blood or bone marrow in EDTA tube
5.0 mL CSF
50.0 mL body fluids
5.0 mL CSF
50.0 mL body fluids
Specimen Handling
Peripheral Blood, Bone Marrow
Collect in Lavender EDTA tube
Mix Gently
Do not centrifuge
Prepare 2 unstained slides
Transport at room temperature
Bone Marrow Core
Transfer Bone Marrow Core to RPMI + 10% FCS
Tightly cap
Transport to laboratory refrigerated within 6 hours
Tissue, Lymph Node, FNA
Transfer to RPMI + 10% FCS
Tightly cap
Transport refrigerated
Body Fluid, CSF
Collect using sterile collection guidelines
Tightly cap
Transport refrigerated
Collect in Lavender EDTA tube
Mix Gently
Do not centrifuge
Prepare 2 unstained slides
Transport at room temperature
Bone Marrow Core
Transfer Bone Marrow Core to RPMI + 10% FCS
Tightly cap
Transport to laboratory refrigerated within 6 hours
Tissue, Lymph Node, FNA
Transfer to RPMI + 10% FCS
Tightly cap
Transport refrigerated
Body Fluid, CSF
Collect using sterile collection guidelines
Tightly cap
Transport refrigerated
Patient Prep
Requires CBC results collected within 3 days for Blood and Bone Marrow Leukemia/Lymphoma panel, clinical history, and differential diagnosis with sample.
Collection Procedure
4.0 mL Lavender Top Tube (EDTA)
Peripheral Blood
Bone marrow
Also acceptable:
6.0 mL Dark Green Top Tube (Sodium Heparin)
Sterile, Leak-Proof Container
Body Fluid
CSF
RPMI + 10% FCS
Tissue
Lymph Node
Bone Marrow Core
FNA
Peripheral Blood
Bone marrow
Also acceptable:
6.0 mL Dark Green Top Tube (Sodium Heparin)
Sterile, Leak-Proof Container
Body Fluid
CSF
RPMI + 10% FCS
Tissue
Lymph Node
Bone Marrow Core
FNA
Unacceptable Conditions
Frozen specimens
Refrigerated peripheral blood
Clotted peripheral blood
Bone Marrow refrigerated in EDTA tube
Tissue in formalin
Note: Clotted samples will be evaluated on an individual basis
Refrigerated peripheral blood
Clotted peripheral blood
Bone Marrow refrigerated in EDTA tube
Tissue in formalin
Note: Clotted samples will be evaluated on an individual basis
Temperature
Ambient (20-26°C)
Refrigerated (2-8°C)
Refrigerated (2-8°C)
Stability
Bone Marrow, Peripheral Blood and Slides:
Ambient: 3 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Bone Marrow Core and Tissue in RPMI:
Ambient: Unacceptable
Refrigerated: 2 days
Frozen: Unacceptable
Body Fluid:
Ambient: Unacceptable
Refrigerated: 3 days
Frozen: Unacceptable
Ambient: 3 days
Refrigerated: Unacceptable
Frozen: Unacceptable
Bone Marrow Core and Tissue in RPMI:
Ambient: Unacceptable
Refrigerated: 2 days
Frozen: Unacceptable
Body Fluid:
Ambient: Unacceptable
Refrigerated: 3 days
Frozen: Unacceptable
Schedule
Monday - Friday
Turn Around Time
Within 1 week
Place of Service
Labcorp
Additional Information
For CD4/CD8 ratio on BAL specimens, order Leukemia Lymphoma Panel (LAB2297) and add "CD4/CD8 ratio on BAL" in the Order Comment
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