T Cell Fx-Complete
Test Code: 831367
Performing Lab: LabCorp
CPT Code: 86353 007
Specimen Type
Whole blood
Specimen Container
Green-top (sodium heparin) tube
Volume
8 mL
Specimen Handling
Specimens must arrive at Viracor-Eurofins within 24 hours collection. Must be drawn between 12-5PM CST Monday through Thursday.
Unacceptable Conditions
Lithium heparin tube; ACD tubes or EDTA anticoagulants.
Temperature
Room temperature
Methodology
Peripheral blood mononuclear cells are stimulated with mitogens or antigens or left unstimulated as a negative control and incubated at 37 degrees C. During the incubation Brefeldin A is added, causing the interferon (IFN)-gamma to be retained inside the cell. Following the stimulation phase, cells are recovered, stained for surface markers (CD45, CD3, and CD69 and intracellular IFN-gamma, and analyzed by flow cytometry.
Stability
Room Temperature: 24 hours
| Result Code | Result Name | LOINC |
|---|---|---|
| 823555 | Viability | UNLOINC |
| 819395 | Unstimulated(Culture)response* | SOLOINC |
| 805288 | Candida albicans Ag response* | SOLOINC |
| 805286 | Phytohemagglutinin(PHA)Mitoge* | SOLOINC |
| 805284 | Pokeweed mitogen(PWM)response* | SOLOINC |
| 805282 | Staph Enterotoxin B (SEB)* | SOLOINC |
| 805280 | Tetanus Toxoid Ag response* | SOLOINC |
| 831375 | Viral Pep. (CMV, EBV, Flu) Ag | SOLOINC |
| 831376 | Viral Prot. (VZV,CMV,Mumps) Ag | SOLOINC |
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