BCR-ABL1 Transcript Detection for Chronic MyelogenousLeukemia and Acute Lymphocytic Leukemia (ALL), Quantitative
Test Code: 480481
Performing Lab: LabCorp
CPT Code: 81206 001 81207 001
Synonym
Acute Lymphocytic Leukemia, BCR-ABL1 Transcript Detection by RT-PCR, Quantitative; Chronic Myelogenous Leukemia, BCR-ABL1 Transcript Detection by RT-PCR, Quantitative; Quantitative BCR-ABL1 Translocation Detection by RT-PCR for CML and ALL
Specimen Type
Whole blood, bone marrow OR cell pellet
Specimen Container
Lavender-top (EDTA) tube, green-top (sodium heparin) tube, yellow-top (ACD-A) tubeTan-top (K2-EDTA) tube, OR Pink-top (K2-EDTA) tube
Volume
3 to 5 mL whole blood OR 1 to 2 mL bone marrow
Minimum Volume
1 mL bone marrow or 3mL whole blood. (Note:This volume does NOT allow for repeat testing).
Specimen Handling
Submit at room temperature. Specimens should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on test request form.
Unacceptable Conditions
Specimen does not meet collection criteria; frozen whole blood or marrow; leaking tube; clotted blood or marrow; grossly hemolyzed; otherwise visibly degraded; contamination by another specimen; containing suspicious foreign material
Temperature
Submit at room temperature. If specimen has to be stored more than 48 hours, refrigerate at 2(degrees)C to 8(degrees)C.
Methodology
Total RNA is isolated from the sample and subject to a real-time, reverse transcriptase polymerase chain reaction (RT-PCR). The PCR primers and probes are specific for BCR-ABL1 e13a2, e14a2 and e1a2 fusion transcripts. The ABL1 transcript is amplified as the control for cDNA quantity and quality. Serial dilutions of a validated positive control RNA with known t(9;22) BCR-ABL1 are used as reference for quantification of BCR-ABL1 relative to ABL1. The numeric BCR-ABL1 level is reported as % BCR-ABL1/ABL1 and the detection sensitivity is 4.5 log below the standard baseline (<0.0032%).
TAT
5 - 7 days
| Result Code | Result Name | LOINC |
|---|---|---|
| 481509 | e13a2 (b2a2) transcript | 52130-2 |
| 481510 | e14a2 (b3a2) transcript | 52131-0 |
| 481516 | e1a2 transcript | 52132-8 |
| 480488 | Interpretation: | 93479-4 |
| 489031 | Director Review | 72486-4 |
| 481507 | Background | 77202-0 |
| 481508 | Methodology | 49549-9 |
| 484516 | References | 52132-8 |
| 511958 | 80563-0 |
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