CDOS

QuantiFERON(R)-TB Gold Plus (Client Incubated)

Test Code: 182893

Performing Lab: LabCorp

CPT Code: 86480 001

Test Link (Copy)

Synonym

Interferon-gamma Release Assay (IGRA) for Mycobacterium tuberculosis; QFT-Plus

Specimen Type

Whole blood

Specimen Container

The QuantiFERON(R)-TB Gold Plus collection kit contains the instructions for the collection and handling of (one each): (1) gray-top (with white ring), uncoated (nil); (2) green cap with white ring, TB1 tube; (3) yellow cap with white ring, TB2 tube; (4) purple top with white ring, mitogen-coated. A high altitude kit is also available for locations between 3350 and 6150 feet; a cap with a yellow ring differentiates the tubes.

Volume

1 mL x four tubes in QuantiFERON-TB Gold Plus kit

Minimum Volume

1 mL x four tubes in QuantiFERON-TB Gold Plus kit

Specimen Handling

Blood collections tubes should be at room temperature at the time of collection. Refer to collection instructions included with draw kit. Special specimen collection kit contains four gel-barrier tubes as noted above. All four tubes are required for a single test result. Each tube is designed to draw only 1 mL and fill time may be longer than other blood collection tubes. Because of the limited vacuum in these tubes, use a needle and holder (not a butterfly) to collect QuantiFERON(R)specimens. If a butterfly is required, first collect other required tubes or use another Vacutainer (R) tube to purge the butterfly line of air and then proceed with drawing the QuantiFERON tubes. Fill tubes to the black fill line on the tube. If tubes are underfilled or overfilled (see kit insert), immediately collect a replacement tube. Following proper fill, label the tubes appropriately and shake tubes 10 times firmly enough to ensure the entire surface of the tube is coated with blood cells to solubilize the antigen on the tube walls. After shaking, the volume may fall below the fill line. Do NOT centrifuge or refrigerate specimens. Return each of the four properly filled, labeled, and shaken tubes to the box labeled "QFT kit." Seal the top by removing tape from the adhesive.

Unacceptable Conditions

Grossly hemolyzed, icteric, or lipemic samples; expired collection tubes; Specimen centrifuged, refrigerated, or frozen; specimen more than 94 hours old on receipt by lab; receipt of three tube collection kit. Under-filled or over-filled tubes; tubes that do not yield sufficient plasma for testing.

Temperature

1. Maintain specimen at room temperature prior to incubation at 37 degrees C. Stability: Kit MUST be incubated within 16 hours of collection. 2. After incubation, maintain specimen at room temperature (17 degrees C to 27 degrees C). Do NOT centrifuge refrigerate, or ship tubes on ice.

Methodology

Whole blood interferon-gamma test measuring responses to CD4+ and CD8+ T cell antigens

Result Code	Result Name	LOINC
182885	QuantiFERON Criteria	8251-1
182886	QuantiFERON TB1 Ag Value	64084-7
182887	QuantiFERON TB2 Ag Value	88517-8
182891	QuantiFERON Nil Value	71776-9
182892	QuantiFERON Mitogen Value	71774-4
182894	QuantiFERON-TB Gold Plus	71773-6

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