Factor 5 Assay
Test Code: FACV
CPT Code: 85220
Test Name
Factor 5 Assay
Epic Test Code
LAB304
Alias
Factor V Activity (Not for Thrombophilia Testing); F5; Labile Factor; Proaccelerin; FV
Specimen Type
One 2.0 mL (min: 1.0 mL) platelet poor plasma frozen
IMPORTANT: CRITICAL FROZEN
IMPORTANT: CRITICAL FROZEN
Specimen Container
Two light blue (3.2% sodium citrate) 2.7 mL
Specimen Handling
Always draw a white discard tube (min: 2.0 mL) prior to blue top tubes.
Separate plasma from cells within 1 hour of collection, then immediately aliquot the plasma from the cells. Transport platelet poor plasma aliquot frozen.
CRITICAL FROZEN.
Separate plasma from cells within 1 hour of collection, then immediately aliquot the plasma from the cells. Transport platelet poor plasma aliquot frozen.
CRITICAL FROZEN.
Unacceptable Conditions
Clotted specimens; hemolyzed specimens (dependent on the level of hemolysis); delayed transport to lab; plasma stored in glass tubes; repeated freeze/thaw cycles; short filled tube (volume of blood varies by more than 10% from indicated fill line on tube)
Methodology
Clotting
Schedule
Daily
Reporting Time
Final: 24 hours
Additional Information
Patient Preparation: Patients with hematocrit values above 55% must have the citrate anticoagulant adjusted prior to the blood collection.
Clinical Significance: Useful for diagnosing congenital deficiencies and acquired deficiencies seen in liver disease, DIC, myeloproliferative disorders, and Factor V inhibitors. Also useful for the evaluation of a prolonged protime or activated partial thromboplastin time (APTT).
Order Remarks: This test measures the activity of Factor V, a liver produced clotting factor. This test does NOT evaluate for Factor V Leiden, a common mutation associated with a predisposed risk of thrombophilia/thrombotic events due to activated protein C resistance. To test for Factor V Leiden mutation, order test Activated Protein C Resistance w/ Reflex (LAB4102).
Clinical Significance: Useful for diagnosing congenital deficiencies and acquired deficiencies seen in liver disease, DIC, myeloproliferative disorders, and Factor V inhibitors. Also useful for the evaluation of a prolonged protime or activated partial thromboplastin time (APTT).
Order Remarks: This test measures the activity of Factor V, a liver produced clotting factor. This test does NOT evaluate for Factor V Leiden, a common mutation associated with a predisposed risk of thrombophilia/thrombotic events due to activated protein C resistance. To test for Factor V Leiden mutation, order test Activated Protein C Resistance w/ Reflex (LAB4102).
Stability
Ambient: Unacceptable / Refrigerated: Unacceptable / Frozen: 2 Weeks (avoid repeated freeze/thaw cycles)
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