CULTURE, URINE
Test Code: LAB239
Synonym
C Bact Urine; Culture Bacteria Urine; Urine Colony Count Culture; C Urine
Specimen Type
Clean-voided midstream urine, catheter, cystoscopic, ileal conduit, or suprapubic aspirate
Alternate Specimen Type
Vacuette urine CCM tube
Specimen Container
Sterile leakproof plastic urine container and then transfer to a urine boric acid tube
Preferred Volume
4 mL
Minimum Collection Volume
3 mL
Specimen Handling
Transfer urine to boric acid tube.
Collection Procedure
Aseptically collect urine. Morning first voided urine is preferred. Note time and method of collection (clean catch, straight cath, foley cath). Foley Catheter: Aspirate through disinfected tubing with a needle and syringe. Do not drain from bag. Place urine in a sterile container and then transfer to a urine boric acid tube. Male Clean Catch: Draw foreskin back (hold in this position until specimen is obtained). Begin voiding, obtain midstream urine specimen in container and transfer to a urine boric acid tube. Female Clean Catch: Separate the folds of the vulva (hold in this position until urine is obtained). Wipe the opening from front to back with four wipes. Use wipe for one stroke only. Obtain mid-stream urine specimen in container and transfer to a urine boric acid tube.
Unacceptable Conditions
Unpreserved urines GT 2 hours at room temperature or GT 1 day refrigerated or GT 2 days preserved at room temperature.
Temperature
Ambient (room temperature) or refrigerated (unpreserved specimens must be refrigerated)
Methodology
Culture
Stability
Room Temp Unpreserved: 2 hours, Preserved: 2 days RefrigeratedUnpreserved: 1 day, Preserved: 2 days Frozen (-20 °C)Unacceptable Frozen (-70 °C) Unacceptable
Schedule
Daily
Turn Around Time
Positive culture reported as soon as detected. 2-5 days
Place of Service
Sacred Heart Medical Center
Department
Microbiology
Additional Information
For inpatients, ordering must be done through the Urinalysis reflex to culture or urine culture Panel. See Link for further instructions. Contact the lab for further questions about ordering. Outpatients please order LAB239 for urine culture.
| Reflex Order Code | Reflex Order Name | Reflex CPT4 |
|---|---|---|
| Organism Identification | Organism Identification | possible additional charges (multiple instances of each CPT possible): 87088, 87184, 87186, 87181, 87147 |
| Susceptibility testing | Susceptibility testing |
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